Journal: Human reproduction (Oxford, England)

Article Title: Activin A regulates trophoblast cell adhesive properties: implications for implantation failure in women with endometriosis-associated infertility.

doi: 10.1093/humrep/deq097

Figure Lengend Snippet: Figure 2 pSmad2 of HTR8 trophoblast cells.(A) Treatment of HTR8 trophoblast cells with activin A (ActA; 1, 10, 50 and 100 ng/ml) for 24 h results in a dose-dependent increase in pSmad2 as shown by western blot. Addition of activin inhibitor, SB431542 (SB: 10 mM) in conjunction with ActA (50 ng/ml), decreased pSmad2 compared with ActA alone. The lower band represents total Smad2 which is not changed with ActA treatment. (B) Densitometric analysis of representative western blot in (A). Each bar represents the mean of two experiments assayed in duplicate.

Article Snippet: Membranes were exposed to autoradiography film (Hyperfilm ECL; GE Healthcare) for 5 min. Membranes were then washed in stripping buffer (Re Blot Plus, by guest on N ovem ber 16, 2015 http://hum rep.oxfordjournals.org/ D ow nloaded from Chemicon International, CA, USA) before blocking again in 10% skim milk/TBS, overnight incubation with rabbit anti-human Smad2 (86F7; Cell Signalling Technology, #3122; 1:1000 in 5% skim milk/TBS) and secondary antibody and detection as described above.

Techniques: Western Blot

Journal: EMBO Reports

Article Title: Promyelocytic leukemia protein targets MK2 to promote cytotoxicity

doi: 10.15252/embr.202052254

Figure Lengend Snippet:

Article Snippet: Rabbit Monoclonal anti‐phospho‐NF‐κB p65 (Ser536) (93H1) , Cell Signaling , #3033, RRID:AB_331284 , WB 1:4,000 (Blocking buffer: Immobilon® Block ‐ PO).

Techniques: Generated, Transgenic Assay, Recombinant, Plasmid Preparation, Blocking Assay, Transfection, Antibody Labeling, Software, Cell Viability Assay